Chronic lymphocytic lymphoma: An overview

Chronic lymphocytic lymphoma (CLL) is a mature B-cell neoplasm characterized by progressive accumulation of monoclonal B lymphocytes.¹ As the most prevalent leukemia in the Western Hemisphere, several novel targeted therapies have been developed for CLL over the past decade, such as Bruton’s tyrosine kinase inhibitors (BTKi), B-cell lymphoma 2 inhibitors (BCL2i), and phosphoinositide 3-kinase inhibitors (PI3Ki). Such therapies have fundamentally changed the therapeutic landscape and significantly improved the prognosis for patients with CLL.¹

Etiology

While the exact cause of CLL is unknown, multiple genetic mutations may contribute to altering the DNA of blood-producing cells; this impacts their function.^1,2 The most common chromosomal aberrations reported in patients with CLL include either deletion or  loss of part of:

• chromosome 11;
• chromosome 13; and/or
• chromosome 17

Parents, siblings, or children of patients with CLL are at a 5–7 times greater risk of developing CLL².

Epidemiology

CLL is considered to be a cancer that (Figure 1)¹

• Occurs in the elderly population, with a median age at diagnosis of ~70 years.
• Varies in incidence by race and geographic location (higher among Caucasians and extremely low in Asia).
• Accounts for approximately 25–35% of all leukemias in the US.

Pathophysiology

The pathophysiology of CLL involves (Figure 2) ⁶

1. Hematopoietic stem cells (HSCs) differentiate into lymphoblasts and proliferate. Mutations that contribute to the development of CLL may happen at this stage and give rise to B cells with survival and growth advantages.
2. Next, the lymphoblasts differentiate into prolymphocytes and proliferate. If no mutations were present in the differentiation of HSCs to lymphoblasts, there is another chance for an oncogenic event to occur here before the lymphoblasts differentiate.
3. In the final stage of CLL cell development, prolymphocytes differentiate into B cells and proliferate.

Once fully differentiated and an oncogenic event has occurred, B cells grow and divide uncontrollably in the bone marrow or the bloodstream causing chronic lymphocytic leukemia cells to develop.

Signs and Symptoms

Patients are often asymptomatic at the time of diagnosis but then develop symptoms, as presented in Figure 3.⁸

Diagnosis

Diagnosis is established by blood counts, blood smears, and immunophenotyping of circulating B-lymphocytes; this can identify clonal B-cell populations carrying the CD5 antigen as well as typical B-cell markers, including CD19/20/23.⁸ In CLL, the levels of surface immunoglobulin CD20 and CD79b are low compared with normal B cells.⁸ The diagnosis of CLL is usually confirmed by ⁸:

• ≥5000 B-lymphocytes/µL in peripheral blood for at least 3 months.
• Clonality of circulating B-lymphocytes confirmed by flow cytometry.
• Small and mature lymphocytes with a narrow border of cytoplasm and dense nucleus lacking distinct nucleoli, and the presence of partially aggregated chromatin confirmed by a blood smear.
• Gumprecht nuclear shadows or smudge cells, found as cell debris.

Risk assessment

There are two staging systems used to determine the stage of disease: the Rai staging system and the Binet staging system.⁹ The stages in the Rai staging system are defined in Figure 4.

The Binet staging system is based on several areas and defined by the presence of enlarged lymph nodes (>1 cm in diameter) or organomegaly, anemia, and thrombocytopenia. The involved areas include the head and neck, axillae, groins, palpable spleen, and liver. The stages in the Binet staging system are outlined in Figure 5.⁹

Prognosis

Whole genome sequencing has enabled characterization of the genomic landscape in CLL, with prognosis varying according to mutational status.¹⁰

• Mutations in the TP53 gene predict resistance to chemoimmunotherapy and a shorter time to progression with most targeted therapies.

• Patients with a del(11q) clone commonly indicates rapid progression, shorter OS, and bulky lymphadenopathy.
• Patients with a del(17p) mutation also present a poor prognosis with low survival rates.

Guidance on diagnosis may vary between countries. Please read below section on key guidelines for further information.

Management

The treatment landscape for CLL has progressed in the last decade. The previously recommended chemotherapeutic agents in combination with a monoclonal antibody have been replaced by targeted agents, such as BTKi and BCL2 inhibitors.¹⁰ Figure 6 summarizes the available types of therapy and their cellular targets.

 

Patients are recommended different treatment options depending on age, comorbidities, and previous lines of therapy.¹⁷. Comorbidities are indicated by the Cumulative Illness Rating Scale rating organs for severity and impairment.¹⁸

Key Guidelines and organizations

The National Comprehensive Cancer Network¹⁹ published updated CLL/SLL guidelines in 2022. These updates were also covered by the Lymphoma Hub.²⁰. In addition, patient support group pages are available from Cancer Research UK²¹ and National Organization for Rare Diseases.²