CD20-directed CAR T-cell therapies in the treatment of relapsed/refractory non-Hodgkin lymphoma: Results from two phase I trials

CD20 is an effective therapeutic target for B-cell non-Hodgkin lymphoma (B-NHL), and the CD20-directed chimeric antigen receptor (CAR) T-cell therapy, C-CAR066, has demonstrated high in vitro and in vivo antitumor activity. C-CAR039 is a novel second generation 4-1BB bispecific CAR T-cell therapy targeting both CD19 and CD20 antigens, and it similarly shows high antitumor activity both in vitro and in vivo. At the 2021 American Society of Clinical Oncology (ASCO) Annual Meeting, Aibin Liang presented initial findings from two trials, one evaluating C-CAR066 (NCT04036019)¹ and the other evaluating C-CAR039 (NCT04317885)².The key findings are presented here.

Study designs^1,2

• A phase I, first-in-human, open-label trial of C-CAR066 was conducted in two sites in China, in patients aged ≥18 years and with relapsed/refractory (R/R) B-NHL after failing CD19 CAR T-cell therapy.
• A phase I, open-label, dose escalation trial of C-CAR039 was conducted in four sites in China, in patients ≥18 years with R/R B-NHL, including diffuse large B-cell lymphoma (DLBCL), follicular lymphoma, and mantle cell lymphoma.
• Patients were given C-CAR066 or C-CAR039 infusion, as shown in Figure 1.
• For both trials, primary endpoints were incidence and severity of treatment emergent adverse events. Secondary endpoints included overall response rate (ORR), duration of response, progression-free survival, and overall survival, and exploratory endpoints were CAR T cell expansion and persistence.

Figure 1. Treatment schema*  

CY, cyclophosphamide; FLU, fludarabine.
*Adapted from Liang, et al.^1,2
†Bridging therapy was permitted.

 Baseline characteristics

The baseline characteristics for the patients included in the C-CAR066¹ and C-CAR039² trials are presented in Table 1.

Table 1. Baseline characteristics*

Results

C-CAR066 anti-CD20 CAR T-cell therapy¹

Safety

• In total, eight patients (80%) had Grade 1–2 cytokine release syndrome (CRS) and one patient (10%) had Grade 4 CRS. The median days to onset and to resolution of CRS were 2 days (range, 1–9 days) and 4 days (range, 2–17 days), respectively.
• Adverse events (AEs) of Grade ≥3 included neutropenia (80%), anemia (50%), thrombocytopenia (30%), and infections (10%).
• Cytopenias were mostly related to cyclophosphamide/fludarabine lymphodepletion and were reversible.
• No immune effector cell-associated neurotoxicity syndrome (ICANS) occurred in any of the patients.

Efficacy

• Patients demonstrated an ORR of 100% and a complete response rate (CRR) of 70%. The median time to complete response (CR) was 2.7 months (range, 0.9–2.9 months) (Table 2).
• Four patients remained in CR after 10 months.
• C-CAR066 proliferation and expansion in the peripheral blood correlated with B-cell depletion.

Table 2. Response rates*

C-CAR039 anti-CD20/CD19 bispecific CAR T-cell therapy²

Safety

• In total, 93% of patients developed CRS, although only 4% of patients had Grade ≥3 CRS (Table 3).
• Median days to onset and resolution of CRS were 2.5 days (range, 0–10 days) and 4 days (range, 1–25 days), respectively (Table 3). Shorter duration of CRS onset and longer duration of resolution were noted in higher dose groups.
• ICANS occurred at Grade 1 in two patients; both were in the 5.0 × 10⁶ CAR-T cell/kg dosing group (Table 3). Infections were common (54%), but Grade 3 infection only occurred in one patient.
• Hematologic Grade ≥3 AEs included leukopenia (89%), neutropenia (89%), lymphopenia (96%), anemia (32%), thrombocytopenia (30%), and infection (4%).
• Other Grade ≥3 AEs were infrequent and included hypertriglyceridemia (4%), hypokalemia (4%), increased alanine aminotransferase (7%), and increased aspartate aminotransferase (7%).

Table 3. CRS and ICANS in patients treated with different doses of C-CAR039

Efficacy

• A total of 27 patients were evaluable for investigator-assessed efficacy. For the overall patient cohort, CRR was 85% and ORR was 93%. Patients with DLBCL had a 92% ORR and an 83% CRR.
• Median time to first response was 1 month (range, 0.9–1.6 months), and median time to CR was 1 month (range, 0.9-6.0 months).
• CRR was consistent across key subgroups.
• Median follow-up was 7 months (range, 1.9–17.2 months) and the progression-free survival rate at 6 months was 83% (95% confidence interval, 69–100). Median duration of response was not reached.
• C-CAR039 proliferation and expansion in the peripheral blood correlated with B-cell depletion.

Conclusion

The novel anti-CD20 CAR T-cell therapy, C-CAR066, demonstrated a favorable safety profile and encouraging clinical benefit in patients with R/R DLBCL following failure of CD19-directed CAR-T cell therapy. Similarly, the anti-CD20/CD19 bispecific CAR T-cell therapy, C-CAR039, demonstrated a satisfactory safety profile and promising efficacy in patients with R/R B-NHL. However, the findings were limited by a small sample size in both studies. Further evaluation in more patients, with a longer follow-up, to confirm safety, efficacy, and duration of response is underway.