All content on this site is intended for healthcare professionals only. By acknowledging this message and accessing the information on this website you are confirming that you are a Healthcare Professional. If you are a patient or carer, please visit the Lymphoma Coalition.

The Lymphoma Hub uses cookies on this website. They help us give you the best online experience. By continuing to use our website without changing your cookie settings, you agree to our use of cookies in accordance with our updated Cookie Policy

An expert panel hosted by

The Lymphoma Hub logo and the Multiple Myeloma Hub logo

Sequencing immune-based therapies in B-cell malignancies

with Ulric Jäger, Sagar Lonial, and Krina Patel

Saturday, June 15 | 18:00-19:30 CEST

Register now

This independent education activity is sponsored by Bristol Myers Squibb. All content is developed independently by the faculty. Funders are allowed no direct influence on the content of this activity.


The Lymphoma Hub website uses a third-party service provided by Google that dynamically translates web content. Translations are machine generated, so may not be an exact or complete translation, and the Lymphoma Hub cannot guarantee the accuracy of translated content. The Lymphoma Hub and its employees will not be liable for any direct, indirect, or consequential damages (even if foreseeable) resulting from use of the Google Translate feature. For further support with Google Translate, visit Google Translate Help.

Steering CommitteeAbout UsNewsletterContact
You're logged in! Click here any time to manage your account or log out.
You're logged in! Click here any time to manage your account or log out.

ASPEN trial | Comparison of zanubrutinib vs ibrutinib in WM with MYD88 mutation and zanubrutinib monotherapy in MYD88 wild-type WM

Aug 20, 2020

Bruton tyrosine kinase (BTK) inhibition is a desired treatment target in Waldenström’s macroglobulinemia (WM) due to its role in B-cell receptor signaling. Zanubrutinib is under investigation as a potent, selective, irreversible, next-generation BTK inhibitor with the aim of a maximum BTK occupancy and a minimum off-target inhibition of TEC- and EGFR-family kinases.

ASPEN (NCT03053440) is a head-to-head phase III trial comparing zanubrutinib versus ibrutinib, a first-generation BTK inhibitor, in patients with WM who have myeloid differentiation primary response gene 88 (MYD88) mutation. BTK inhibitors have demonstrated significant activity in the presence of MYD88 gene mutation but response rates and progression-free survival (PFS) lower when it is lacking. Investigators also included a second cohort of patients with MYD88 wild-type WM or with unknown mutations to evaluate the efficacy and safety of zanubrutinib monotherapy. Dimopoulos and colleagues presented their results from the two different cohorts during the American Society of Clinical Oncology (ASCO) 2020 Virtual Annual Meeting1 and the Virtual Edition of the 25th European Hematology Association (EHA) Annual Congress.2,3 Here, we provide a summary of the results combined.

The U.S. Food and Drug Administration (FDA) has announced the accelerated approval of zanubrutinib for patients with mantle cell lymphoma, and very recently, the European Medicines Agency (EMA) has accepted marketing authorization application for the treatment of WM based on the results of the ASPEN trial4.

Study Design1–3

Eligibility criteria included:1

  • histological diagnosis of WM
  • meeting ≥ 1 criterion for treatment initiation*
  • being unsuitable for standard chemoimmunotherapy for treatment-naïve patients, and
  • no previous treatment with BTK inhibitors

*Clinical indications: recurrent fever, night sweats, weight loss, fatigue, hyperviscosity, symptomatic or bulky lymphadenopathy, symptomatic hepatomegaly, splenomegaly, organomegaly, WM-related peripheral neuropathy. Laboratory indications: symptomatic cryoglobulinemia, cold agglutinin anemia, immune hemolytic anemia or thrombocytopenia, WM-related nephropathy or amyloidosis, hemoglobin 19 g/dL, platelet count < 100 × 109/L.5

Cohort 1 (comparison cohort) included 201 patients (of which 164 were R/R) with the MYD88 mutation, stratified by C-X-C motif chemokine receptor 4 (CXCR4) status and the number of prior lines of therapy, and randomized in a 1:1 fashion to receive either1,2

  • zanubrutinib 160 mg twice daily until progression (n = 102)
  • ibrutinib 420 mg once daily until progression (n = 99)

Cohort 2 included 28 patients (of which 23 were R/R) with MYD88 wild-type WM who were treated with zanubrutinib 160 mg twice daily until progression.1,3

The primary objective was to compare the efficacy of zanubrutinib versus ibrutinib based on complete response (CR) or very good partial response (VGPR) in Cohort 1. Secondary objectives were to compare clinical benefit, anti-lymphoma effects, and safety.1,2

Patient characteristics1–3

Cohort 1

  • Patient characteristics were well balanced between the two groups1,2
  • Median age was 70 years in both treatment arms; the number of patients > 75 years of age was greater in the zanubrutinib arm (see Table 1) 1,2
  • The number of patients who received 0, 1–3, or > 3 previous lines of therapy, or who were MYD88L265P/CXCR4WT, were similar across both arms1,2
  • More patients had anemia in the zanubrutinib arm1,2

Table 1. Patient and disease characteristics across both arms (intent-to-treat population)1,2

CXCR4, C-X-C motif chemokine receptor 4; IPSS WM, International Prognostic Scoring System for Waldenström’s Macroglobulinemia; MYD88, myeloid differentiation primary response gene 88; WT, wild type



(n = 99)


(n = 102)

Age, n (%)

> 65 years

> 75 years


70 (70.7)

22 (22.2)


61 (59.8)

34 (33.3)

Gender, n (%)




65 (65.7)

34 (34.3)


69 (67.6)

33 (32.4)

Previous lines of therapy, n (%)



> 3


18 (18.2)

74 (74.7)

7 (7.1)


19 (18.6)

76 (74.5)

7 (6.9)

Genotype, n (%)




90 (90.9)

8 (8.1)


91 (89.2)

11 (10.8)

IPSS WM, n (%)





13 (13.1)

42 (42.4)

44 (44.4)


17 (16.7)

38 (37.3)

47 (46.1)

Hemoglobin 110 g/L, n (%)

53 (53.5)

67 (65.7)

Cohort 2

  • The median age was 72 years; 42.9% of patients were over 75 years old3
  • Most patients (n = 23) were relapsed/refractory with at least one previous therapy and had MYD88 wild-type disease (n = 26)3
  • The proportion of patients with intermediate- and high-risk disease was 39.3% and 42.9%, respectively3


Cohort 1

By cutoff date of August 31, 20191,2,

  • the median follow-up was 19.4 months
  • there was no statistically significant difference in CR + VGPR between the two arms (p = 0.09), indicating that the trial did not meet its primary endpoint. As CR is very uncommon in WM, investigators compared VGPR rates in both arms (see Figure 1)

Figure 1. Treatment response across treatment arms1,2

ITT, intent to treat; PR, partial response; VGPR, very good partial response

The difference in VGPR rates assessed by the investigators was statistically significant in the original  August 2019 cutoff date (17.2% in the ibrutinib arm vs 28.4% in the zanubrutinib arm; p = 0.04) and at a later cutoff date of January 2020 (18.2% in the ibrutinib arm vs 30.4% in the zanubrutinib arm; p = 0.03). In addition, immunoglobulin M reduction over time was significantly greater in the zanubrutinib arm compared with the ibrutinib arm (p = 0.037).

The PFS and overall survival (OS) probability at 12 months were similar across the zanubrutinib and ibrutinib arms:

  • PFS: 89.7% vs 87.2%, respectively
  • OS: 97.0% vs 93.9%, respectively

Patients in the zanubrutinib arm with R/R disease tended to have longer PFS and OS at the 12-month time point compared with ibrutinib:

  • PFS: 92.4% vs 85.9%, respectively
  • OS: 98.8% vs 92.5%, respectively


The number of patients experiencing at least one adverse event (AE) or serious AEs were similar between the two arms. Key safety findings for Cohort 1 are summarized in Table 2.

Table 2. Adverse event rates across treatment arms1, 2

AE, adverse event

*p value < 0.05.

Category, n (%)


(n = 98)


(n = 101)

Grade 3 AEs

62 (63.3)

59 (58.4)

Serious AEs

40 (40.8)

40 (39.6)

AE leading to


treatment discontinuation

dose reduction

dose held


4 (4.1)

9 (9.2)

23 (23.5)

55 (56.1)


1 (1.0)

4 (4.0)

14 (13.9)

47 (46.5)

1 treatment-related AE

84 (85.7)

80 (79.2)

1 AE of interest

81 (82.7)

86 (85.1)

Most common Grade ≥ 3 AEs (≥ 5%)




atrial fibrillation/flutter*



8 (8)

11 (11)

7 (7)

4 (4.1)

8 (8.2)


16 (16)

6 (6)

1 (1)

0 (0.0)

6 (5.9)

Updated safety data show a greater difference in atrial fibrillation/flutter rate in favor of zanubrutinib (0% vs 7.1% in the ibrutinib arm), but neutropenia rates increased to 22.8% in the zanubrutinib arm (vs 8.2% in the ibrutinib arm). However, the greater number of neutropenia events did not lead to a higher rate of infections with zanubrutinib as the infection rate was similar between both arms (18.8% for zanubrutinib vs 23.5% for ibrutinib). As patients responded well to both treatments, quality of life improved; but zanubrutinib led to better quality of life scores, which was attributed to better tolerability.

Cohort 2

Median follow-up was 17.9 months. Out of the 28 patients, two patients discontinued treatment due to AEs. None of the patients achieved a complete response. Patients with unknown mutation status achieved partial response.3 Key findings are summarized in Table 3.

Table 3. Best overall response rates in patients with MYD88 wild-type WM3

PR, partial response; R/R, relapsed/refractory; SD, stable disease; TN, treatment-naïve; VGPR, very good partial response

Outcome, n (%)


(n = 5)


(n = 21)


(n = 26)


1 (20.0)

6 (28.6)

7 (26.9)


1 (20.0)

5 (23.8)

6 (23.1)

Minor response

2 (40.0)

6 (28.6)

8 (30.8)


1 (20.0)

3 (14.3)

4 (15.4)



1 (4.8)

1 (3.8)

Overall response rate

4 (80.0)

17 (81.0)

21 (80.8)

PFS event-free survival at 12 months was 72.4%.

Safety analysis showed that the most common AEs included diarrhea, anemia, contusion, pyrexia, and upper respiratory tract infection. While one patient experienced atrial fibrillation, two patients experienced major hemorrhage.


The results from the comparison cohort demonstrate that the primary objective of study was not met, with similar rates of CR + VGPR across two arms, but secondary endpoints, including investigator-assessed response rates and immunoglobulin M reduction over time, favored zanubrutinib. PFS and OS rates were also comparable, with both treatments associated with favorable PFS and OS. The safety profile and tolerability of zanubrutinib were considered better based on lower rates of AEs leading to death, treatment discontinuation, or interruptions, and statistically significant lower rates of atrial fibrillation/flutter, hypertension, and pneumonia.

Zanubrutinib demonstrated clinically meaningful antitumor activity in the MYD88 wild-type WM cohort, with high response rates and a favorable safety profile based on low rates of treatment discontinuation.

  1. Tam CSL, Opat S, D'Sa S, et al. ASPEN: Results of a phase III randomized trial of zanubrutinib versus ibrutinib for patients with Waldenström macroglobulinemia (WM). Oral abstract #8007. 2020 ASCO Virtual Scientific Program; Virtual.
  2. Dimopoulos M, Opat, S, D'Sa S, et al. ASPEN: Results of a phase 3 randomized trial of zanubrutinib versus ibrutinib for patients with Waldenström macroglobulinemia (WM). Oral abstract #S225. 25th EHA Annual Congress; Jun 12, 2020; Virtual.
  3. Dimopoulos M, Sanz RG, Lee H-P, et al. Updated results of the ASPEN trial from a cohort of patients with MYD88 wild-type Waldenström macroglobulinemia. Poster #EP1180. 25th EHA Annual Congress; Jun 12, 2020; Virtual.
  4. Global News Wire. BeiGene Announces European Medicines Agency Acceptance of its Marketing Authorization Application for BRUKINSA® (Zanubrutinib) for the Treatment of Patients with Waldenström’s Macroglobulinemia. Published Jun 18, 2020. Accessed Aug 20, 2020.
  5. Dimopoulos MA, Kastritis E, Owen RG, et al. Treatment recommendations for patients with Waldenström macroglobulinemia (WM) and related disorders: IWWM-7 consensus. Blood. 2014;124(9):1404-1411. DOI: 10.1182/blood-2014-03-565135

Understanding your specialty helps us to deliver the most relevant and engaging content.

Please spare a moment to share yours.

Please select or type your specialty

  Thank you


Subscribe to get the best content related to lymphoma & CLL delivered to your inbox